This Human Visceral Adipose-Specific Serpin (Serpin A12) ELISA Kit from Innovative Research is intended for quantitative detection of human Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA) and urine. Strip well format. Reagents for up to 96 tests.

This human Serpin A12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin A12 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L21-K414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Visceral Adipose-Specific Serpin (Serpin A12)
• Uniprot ID: Q8IW75)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information:

The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: This gene is mapped to 14q32.13.; Immunogen sequence: 333

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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