This Human Tumor Necrosis Factor Ligand Superfamily Member 10 (TRAIL) Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and saliva. Strip well format. Reagents for up to 96 tests.
This human TRAIL Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAIL has been precoated onto 96-well plates. Standards (NSO,T95-G281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAIL amount of sample captured in plate.
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Detection Target: Tumor Necrosis Factor Ligand Superfamily Member 10 (TRAIL)
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Uniprot ID: P50591)
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Reactivity: Human
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 15.6pg/ml-1000pg/ml
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Sensitivity: <1pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.; Immunogen sequence: 400
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