Human Tubulointerstitial Nephritis Antigen-Like (TINAGL1) ELISA Kit

This Human Tubulointerstitial Nephritis Antigen-Like (TINAGL1) ELISA Kit from Innovative Research is intended for quantitative detection of human TINAGL1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.

This human TINAGL1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TINAGL1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A22-H467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TINAGL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TINAGL1 amount of sample captured in plate.

• Detection Target: Tubulointerstitial Nephritis Antigen-Like (TINAGL1)
• Uniprot ID: Q9GZM7)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tubulointerstitial nephritis antigen-like is a protein that in humans is encoded by the TINAGL1 gene. It is mapped to 1p35.2. The protein encoded by this gene is similar in sequence to tubulointerstitial nephritis antigen, a secreted glycoprotein that is recognized by antibodies in some types of immune-related tubulointerstitial nephritis. Three transcript variants encoding different isoforms have been found for this gene.; Immunogen sequence: 369