Human Transcobalamin 2 ELISA Kit

This Human Transcobalamin 2 ELISA Kit from Innovative Research is intended for quantitative detection of human TCN2 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Strip well format. Reagents for up to 96 tests.

This human TCN2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TCN2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E19-W427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TCN2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TCN2 amount of sample captured in plate.

• Detection Target: Transcobalamin 2
• Uniprot ID: P20062)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 0.78ng/ml-50ng/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.; Immunogen sequence: 369