Human Sialic Acid-Binding Ig-Like Lectin 5 (Siglec-5) ELISA KitIHUSIGLEC5KT
This Human Sialic Acid-Binding Ig-Like Lectin 5 (Siglec-5) ELISA Kit from Innovative Research is intended for quantitative detection of human Siglec5 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human Siglec5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Siglec5 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E17-L441) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Siglec5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Siglec5 amount of sample captured in plate.
- Detection Target: Sialic Acid-Binding Ig-Like Lectin 5 (Siglec-5)
- Uniprot ID: O15389)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 46.9pg/ml-3000pg/ml
- Sensitivity: <15pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Sialic acid-binding Ig-like lectin 5 is a protein that in humans is encoded by the SIGLEC5 gene. This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.; Immunogen sequence: 369
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.
Get in touch with our team! Please include any relevant information (product name, etc)