Human Serum Paraoxonase/Arylesterase 1 ELISA KitIHUPON1KT
This Human Serum Paraoxonase/Arylesterase 1 ELISA Kit from Innovative Research is intended for quantitative detection of human PON1 in cell culture supernates, cell lysates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.
This human PON1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PON1 has been precoated onto 96-well plates. Standards(NSO, A2-L355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PON1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PON1 amount of sample captured in plate.
- Detection Target: Serum Paraoxonase/Arylesterase 1
- Uniprot ID: P27169)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 31.2pg/ml-2000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).; Immunogen sequence: 369
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