This Human Prorelaxin H3 (Relaxin 3) ELISA Kit from Innovative Research is intended for quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This human Relaxin 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 3 has been precoated onto 96-well plates. Standards(E. coli, D119-C142 (A) & A27-R53(B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 3 amount of sample captured in plate.

• Detection Target: Prorelaxin H3 (Relaxin 3)
• Uniprot ID: Q8WXF3)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.; Immunogen sequence: 369

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