Human Procollagen C-Endopeptidase Enhancer 1 ELISA Kit

This Human Procollagen C-Endopeptidase Enhancer 1 ELISA Kit from Innovative Research is intended for quantitative detection of human PCOLCE in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human PCOLCE ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PCOLCE has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q26-D449) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCOLCE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PCOLCE amount of sample captured in plate.

• Detection Target: Procollagen C-Endopeptidase Enhancer 1
• Uniprot ID: Q15113)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated monoclonal antibody from goat. Expression system for standard: Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. It is mapped to 7q22.1. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. ; Immunogen sequence: 369