Human Peroxiredoxin 2 ELISA Kit

Human Peroxiredoxin 2 ELISA Kit

IHUPRDX2KT

Innovative Research

  • $744.00


This Human Peroxiredoxin 2 ELISA Kit from Innovative Research is intended for quantitative detection of human Peroxiredoxin 2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human Peroxiredoxin 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Peroxiredoxin 2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A2-N198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Peroxiredoxin 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Peroxiredoxin 2 amount of sample captured in plate.

  • Detection Target: Peroxiredoxin 2
  • Uniprot ID: P32119)
  • Reactivity: Human
  • Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
  • Range: 312pg/ml-20000pg/ml
  • Sensitivity: <10pg/ml
  • Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. It may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.; Immunogen sequence: 369

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Product Disclaimer

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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