Human Neutrophil Gelatinase-Associated Lipocalcin (Lipocalin-2) ELISA KitIHUNGALKT
Human Neutrophil Gelatinase-Associated Lipocalcin (Lipocalin-2) ELISA Kit from Innovative Research are highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Human Lipocalin-2 / Neutrophil Gelatinase-Associated Lipocalcin in biological samples. Strip well format. Reagents for up to 96 tests.
In this assay the Lipocalin-2/NGAL present in samples reacts with the anti-Lipocalin-2/NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Lipocalin-2/NGAL antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Lipocalin2/NGAL. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Lipocalin-2/NGAL in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Lipocalin-2/NGAL in the test sample. The quantity of Lipocalin-2/NGAL in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
- Detection Target: Neutrophil Gelatinase-Associated Lipocalcin (Lipocalin-2)
- Reactivity: Human
- Range: 0.625 ng/ml - 20 ng/ml
- Sensitivity: 0.177 ng/ml
- Storage Conditions: 4C
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