This Human Neurotrophic Tyrosine Kinase Receptor Type 3 (TrkC) ELISA Kit from Innovative Research is intended for quantitative detection of human TRKC in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human TRKC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRKC has been precoated onto 96-well plates. Standards(NS0,C32-A428) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRKC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRKC amount of sample captured in plate.

• Detection Target: Neurotrophic Tyrosine Kinase Receptor Type 3 (TrkC)
• Uniprot ID: Q16288)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tropomyosin receptor kinase C (TrkC), also known as NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, or TrkC tyrosine kinase is a protein that in humans is encoded by the NTRK3 gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. ; Immunogen sequence: 369

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