Human Neurogenic Locus Notch Homolog Protein 1 (Notch-1) ELISA Kit

This Human Neurogenic Locus Notch Homolog Protein 1 (Notch-1) ELISA Kit from Innovative Research is intended for Quantitative Detection of human NOTCH1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human NOTCH1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NOTCH1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A19-Q526) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOTCH1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NOTCH1 amount of sample captured in plate.

• Detection Target: Neurogenic Locus Notch Homolog Protein 1 (Notch-1)
• Uniprot ID: P46531)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia.; Immunogen sequence: 399