Human Matrix Metalloproteinease-1 (Interstitial Collagenase) ELISA KitIHUMMP1KT
This Human Matrix Metalloproteinease-1 (Interstitial Collagenase) ELISA Kit from Innovative Research is intended for quantitative detection of human MMP-1 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.
This human MMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-1 has been precoated onto 96-well plates. Standards(NSO, F20-N469) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-1 amount of sample captured in plate.
- Detection Target: Matrix Metalloproteinease-1 (Interstitial Collagenase)
- Uniprot ID: P03956)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 156pg/ml-10000pg/ml
- Sensitivity: <20pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.; Immunogen sequence: 380
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