Human Leucine-Rich Repeats/Immunoglobulin-Like Domains Protein 3 ELISA Kit

This Human Leucine-Rich Repeats/Immunoglobulin-Like Domains Protein 3 ELISA Kit from Innovative Research is intended for quantitative detection of human LRIG3 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.

This human LRIG3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LRIG3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D28-T807) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LRIG3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LRIG3 amount of sample captured in plate.

• Detection Target: Leucine-Rich Repeats/Immunoglobulin-Like Domains Protein 3
• Uniprot ID: Q6UXM1)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.; Immunogen sequence: 369