Human Lanimostim (M-CSF) High-Sensitivity ELISA Kit

This Human Lanimostim (M-CSF) High-Sensitivity ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of Human M-CSF. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests. This human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli, E33-S190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human M-CSF amount of sample captured in plate. Detection Target: Lanimostim (M-CSF) Uniprot ID: P09603) Reactivity: Human Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 62.5pg/ml-4000pg/ml Sensitivity: <10pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice) Additional Information: The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.; Immunogen sequence: 320