Human Interleukin-2 Receptor Alpha Chain (CD25) ELISA Kit
IHUIL2RAKTThis Human Interleukin-2 Receptor Alpha Chain (CD25) ELISA Kit from Innovative Research is intended for quantitative detection of human CD25 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-C213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD25 amount of sample captured in plate.
- Detection Target: Interleukin-2 Receptor Alpha Chain (CD25)
- Uniprot ID: P01589)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 78pg/ml-5000pg/ml
- Sensitivity: <5pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.; Immunogen sequence: 369
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