This Human Interleukin-15 Receptor Alpha (IL-15Ra) Sandwich ELISA Kit from Innovative Research is intended for Quantitative Detection of human IL15RA in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. This human IL15RA Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL15RA has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: I31-T172) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL15RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL15RA amount of sample captured in plate. Detection Target: Interleukin-15 Receptor Alpha (IL-15Ra) Uniprot ID: Q13261) Reactivity: Human Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 156pg/ml-10000pg/ml Sensitivity: <10pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.; Immunogen sequence: 369
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