Human Interleukin-1 Receptor-Like 1 Rapid ELISA Kit

Human Interleukin-1 Receptor-Like 1 Rapid ELISA Kit

IHUIL1RL1KTR

Innovative Research

  • $822.00


This Human Interleukin-1 Receptor-Like 1 Rapid ELISA Kit from Innovative Research is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Human St2/IL1RL1 with <10pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. St2/IL1RL1 tissue specificity: Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not is a fast ELISA kit, this assay takes less than 1.5 hours to detected in brain, heart, liver, kidney and skeletal muscle. . Strip well format. Reagents for up to 96 tests.

This human IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1RL1 has been precoated onto 96-well plates. Standards(NSO, K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1RL1 amount of sample captured in plate.

  • Detection Target: Interleukin-1 Receptor-Like 1
  • Uniprot ID: Q01638)
  • Reactivity: Human
  • Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
  • Range: 156pg/ml-10,000pg/ml
  • Sensitivity: <10pg/ml
  • Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.; Immunogen sequence: 449

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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