Human Fc Receptor-Like Protein 5 ELISA Kit

This Human Fc Receptor-Like Protein 5 ELISA Kit from Innovative Research is intended for quantitative detection of human FCRL5 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human FCRL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FCRL5 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q16-G851) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FCRL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Fc Receptor-Like Protein 5
• Uniprot ID: Q96RD9)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 0.78ng/ml-50ng/ml
• Sensitivity: <0.1ng/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.; Immunogen sequence: 333