This Human Complement Factor H-Related Protein 5 (CFHR5) ELISA Kit from Innovative Research is intended for quantitative detection of human CFHR5 in cell culture supernates, serum and plasma (heparin, EDTA) and urine. Strip well format. Reagents for up to 96 tests.
This human CFHR5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFHR5 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E19-E569) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFHR5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
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Detection Target: Complement Factor H-Related Protein 5 (CFHR5)
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Uniprot ID: Q9BXR6)
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Reactivity: Human
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 0.78ng/ml-50ng/ml
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Sensitivity: <0.1ng/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).; Immunogen sequence: 333
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