Human CMRF35-Like Molecule 1 (CD300LF) ELISA Kit

This Human CMRF35-Like Molecule 1 (CD300LF) ELISA Kit from Innovative Research is intended for quantitative detection of human CD300F in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This mouse CD300F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD300F has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: T20-S156) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD300F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: CMRF35-Like Molecule 1 (CD300LF)
• Uniprot ID: Q8TDQ1)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.; Immunogen sequence: 369