This Human Cluster of Differentation 163 (CD163) Rapid ELISA Kit from Innovative Research is intended is a fast ELISA kit, this assay takes less than 1.5 hours to detect Human CD163 with <150pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma (heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CD163 tissue specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform is a fast ELISA kit, this assay takes less than 1.5 hours to detected in the blood. . Strip well format. Reagents for up to 96 tests.
This human CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD163 has been precoated onto 96-well plates. Standards(NSO, G41-S1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD163 amount of sample captured in plate.
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Detection Target: Cluster of Differentation 163 (CD163)
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Uniprot ID: Q86VB7)
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Reactivity: Human
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 1.56ng/ml-100ng/ml
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Sensitivity: <150pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. Expression system for standard: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.; Immunogen sequence: 419
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