Human Chemokine Ligand CXCL11 ELISA Kit

This Human Chemokine Ligand CXCL11 ELISA Kit from Innovative Research is intended for quantitative detection of human CXCL11 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human CXCL11 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL11 has been precoated onto 96-well plates. Standards(E.coli, F22-F94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL11 amount of sample captured in plate.

• Detection Target: Chemokine Ligand CXCL11
• Uniprot ID: O14625)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Chemokine(C-X-C motif) ligand 11(CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant(I-TAC) and Interferon-gamma-inducible protein 9(IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.; Immunogen sequence: 333