Human C-Type Lectin Domain Family 11 Member A (CLEC11A) ELISA Kit

This Human C-Type Lectin Domain Family 11 Member A (CLEC11A) ELISA Kit from Innovative Research is intended for quantitative detection of human CLEC11A in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human CLEC11A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CLEC11A has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A22-F323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CLEC11A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: C-Type Lectin Domain Family 11 Member A (CLEC11A)
• Uniprot ID: Q9Y240)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. It is mapped to 19q13.33.; Immunogen sequence: 333