Human Bone Morphogenetic Protein 4 ELISA KitIHUBMP4KT
This Human Bone Morphogenetic Protein 4 ELISA Kit from Innovative Research is intended for quantitative detection of human BMP-4 in bone tissue and cell culture supernates. Strip well format. Reagents for up to 96 tests.
This human BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-4 has been precoated onto 96-well plates. Standards(NSO, S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-4 amount of sample captured in plate.
- Detection Target: Bone Morphogenetic Protein 4
- Uniprot ID: P12644)
- Reactivity: Human
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 62.5pg/ml-4000pg/ml
- Sensitivity: <4pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.; Immunogen sequence: 400
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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