Human Apolipoprotein A1 (Apo-A1) ELISA KitIHUAPOA1KT
Human Apolipoprotein A1 (Apo-A1) ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring total Human Apolipoprotein A1 (Apo-A1) in biological samples. Strip well format. Reagents for up to 96 tests.
In this assay the Apolipoprotein A-1 present in samples reacts with the anti-Apolipoprotein A-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Apolipoprotein A-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Apolipoprotein A-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Apolipoprotein A-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Apolipoprotein A-1 in the test sample. The quantity of Apolipoprotein A-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
- Detection Target: Apolipoprotein A1 (Apo-A1)
- Reactivity: Human
- Range: 15.6 ng/ml - 500 ng/ml
- Sensitivity: 4.022 ng/ml
- Storage Conditions: 4C
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