This Human Angiopoietin-Related Protein 2 ELISA Kit from Innovative Research is intended for quantitative detection of human ANGPTL2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human ANGPTL2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q23-H493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL2 amount of sample captured in plate.

• Detection Target: Angiopoietin-Related Protein 2
• Uniprot ID: Q9UKU9)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 312pg/ml-20000pg/ml
• Sensitivity: <50pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.; Immunogen sequence: 369

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