Human 4 Alpha Hydroxysteroid Dehydrogenase (AKR1C4) ELISA Kit

This Human 4 Alpha Hydroxysteroid Dehydrogenase (AKR1C4) ELISA Kit from Innovative Research is intended for quantitative detection of human AKR1C4 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human AKR1C4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AKR1C4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: M1-Y323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AKR1C4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human AKR1C4 amount of sample captured in plate.

• Detection Target: 3 Alpha Hydroxysteroid Dehydrogenase (AKR1C4)
• Uniprot ID: P17516
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: 3-Alpha-Hydroxysteroid dehydrogenase (3-Alpha-HSD or aldo-keto reductase family 1 member C4) is an enzyme that in humans is encoded by the AKR1C4 gene. It is mapped to 10p15.1. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. ; Immunogen sequence: 369