Horse Heparin Binding Growth Factor 1 (FGF1) High-Sensitivity ELISA Kit
IHSFGF1KTHThis Horse Heparin Binding Growth Factor 1 (FGF1) High-Sensitivity ELISA Kit from Innovative Research is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of horse equine FGF1. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Equine FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A2-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine FGF1 amount of sample captured in plate.
- Detection Target: Heparin Binding Growth Factor 1 (FGF1)
- Uniprot ID: P05230)
- Reactivity: Horse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 31.2pg/ml-2000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization. ; Immunogen sequence: 333
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