Canine Serum Amyloid A Sandwich ELISA Kit from Innovative Research s a highly sensitive two-site enzyme linked immunoassay (Sandwich ELISA) for measuring Canine Serum Amyloid A in biofluid samples. Strip well format. Reagents for up to 96 tests. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP.) are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Serum Amyloid A Reactivity: Canine Range: 6.25 ng/ml - 200 ng/ml Sensitivity: Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
Curious About The Best Way To Thaw Plasma?
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results
Click here to view a video tutorial covering frequently asked questions regarding ELISA workflows.
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