Mouse Anti Human IgE Monoclonal Clone 24A HRP Labeled from Innovative Research is a monoclonal antibody in Liquid format, buffered in Phospate buffered saline, pH 7.2 with 0.1% sodium azide as a preservative. Mice were immunized with highly purified human IgE. Splenocytes were collected and fused with SP20 cells to form hybridomas. The IgG2A kappa antibody was then purified from tissue culture fluid using Protein A chromatography and the resulting antibodies were HRP conjugated.

More Details:

• Host: Mouse
• Species: Human
• Target: anti-Human IgE
• Purification: Affinity Purified
• Clonality: Monoclonal Clone 24A
• Storage Conditions: 4C

This product can be used for a wide range of research applications, including ELISA: 2 ug/ml, IP: 2 ug/mg, WB: 0.5 - 0.05 ug/ml. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

• Preheat a water bath to 37 C.
• Place the frozen plasma sample directly in the water bath.
• Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
• The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
• The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
• Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
• Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
• Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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