Mouse Anti Human Serum Amyloid A Monoclonal Clone 1E2 from Innovative Research is a monoclonal antibody in Liquid format, buffered in PBS with 0.1% sodium azide as a preservative. This mouse IgG2a kappa antibody is purified from tissue culture media using salt fractionation procedures and Protein A chromatography. More Details: Host: Mouse Species: Human Target: anti-Human SAA Protein Purification: Purified Protein Clonality: Monoclonal Clone 1E2 Storage Conditions: -20C Sample type: Milk, Plasma, Serum Aliquot and store at -20║C when storing for longer than 14 days, avoid multiple freezethaw cycles. Keeping the product at 4║C is suitable for storing less than 14 days. When tested by immunoelectrophoresis as an antigen concentration of 3.0 mg/ml, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat anti-mouse IgG, Fc fragment specific. By indirect ELISA and Western Blot this product reacts with native and recombinant human SAA. It is suitable to use as the capture antibody when paired with itself or it functions as the detection antibody when using IMSAHUSAA1B4C for capture in sandwich ELISA. Optimal working dilutions should be determined for the particular assay conditions.This product can be used for a wide range of research applications, including ELISA: Assay Dependent, IF: 10 - 2 ug/ml, WB: Assay Dependent. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
Curious About The Best Way To Thaw Plasma?
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results
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