Mouse Anti Feline Serum Amyloid A Monoclonal Clone 3A5 Lyophilized from Innovative Research is a monoclonal antibody in Lyophilized format, buffered in PBS buffer with non-protein stabilizer. This mouse IgG1 kappa is purified using salt fractionation procedures and Protein A chromatography. It is produced from tissue culture media obtained at our facility in The United States.

More Details:

• Host: Mouse
• Species: Feline
• Target: anti-Feline SAA Protein
• Purification: Purified Protein
• Clonality: MonoclonalClone 3A5
• Storage Conditions: -20C

Lyophilized material should be kept at 4°C until rehydrated and will remain stable for a minimum of 2 years. Rehydrate by adding 1.0 mL of reagent quality water to the product vial. Store the liquid solution at 4°C for up to a week; thereafter, aliquots should be prepared and stored frozen. Stable for a minimum of 1 year at -20°C. When tested by immunoelectrophoresis as an antigen at 10 ug per well, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat antimouse IgG Fc fragment specific. By indirect ELISA this antibody reacts with cat SAA. Optimal working dilutions should be determined for the particular assay conditions.This product can be used for a wide range of research applications, including ELISA: Assay Dependent, WB: Assay Dependent. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

• Preheat a water bath to 37 C.
• Place the frozen plasma sample directly in the water bath.
• Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
• The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
• The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
• Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
• Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
• Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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