*Human PAI-1 Recombinant Stable Mutant Low Endotoxin Lyophilized

Select this product for Molecular Innovations name, Human PAI-1 (stable mutant), Low Endotoxin and product number, CPAI-ED.

Human PAI-1 Recombinant Stable Mutant Low Endotoxin from Innovative Research has been recombinantly produced in E. coli and purified by a affinity chromatography methods. This is a Lyophilized powder buffered in0.0067M Phosphate; 0.15M NaCl; pH 7.4 and a purity of >95% as determined by SDS-PAGE.

More Details:

• Species: Human
• Target: PAI-1
• Purification Method: Affinity purified
• Purity: >95% as determined by SDS-PAGE Analysis
• Source: E. coli
• Storage Conditions: -20 C

Additional Information:
The human form of PAI-1 contains the following four mutations: K154T, Q319L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking in into the active conformation. It is an ideal choice for in vivo and cell culture studies. This preparation is purified to lower levels of endotoxin (<0.01 EU/ug) and does not contain animal carrier proteins or sodium azide. It is lyophilized and fully active. Add sterile endotoxin free deionized water to original volume or PAI-1 storage buffer (0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6) to desired volume. Aliquot and freeze unused portion.

This product can be used as a positive control for ELISA and western blot and for a variety of in vitro , in vivo, and cell culture applications including affinity purification, blocking, fibrinolysis, hypertension studies, inhibition, plasminogen activation, protein-protein interactions, receptor binding, and more. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.