*Human Pai 1 Recombinant P9 Nbd LabeledIHUPAI1RP9NBD500UG
Select this product for Molecular Innovations name, Human PAI-1 NBD labeled at P9 of the reactive center loop and product number, HPAI-P9NBD.
Human PAI-1 Recombinant P9-NBD Labeled from Innovative Research has been recombinantly produced in E. coli and purified by affinity chromatography. This is a Frozen liquid buffered in 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 with a concentration of 2.5 mg/ml and a purity of >95% as determined by SDS-PAGE.
- Species: Human
- Target: PAI-1
- Purification Method: Affinity purified
- Purity: >95% as determined by SDS-PAGE Analysis
- Source: E. coli
- Storage Conditions: -20C
Created by mutagenesis of P9 serine residue (Ser338) to cysteine on the reactive center loop, providing a free thiol group for NBD labeling. Fluorescence emission is enhanced upon insertion of the reactive center loop into beta-sheet A (following the formation of complexes with proteinases, formation of the latent species, or cleavage by elastase). This modified version of PAI-1 is nearly as active as wild type PAI-1, but is more resistant to spontaneous latency reaction (making it suitable in applications like monitoring reaction rates of PAI-1).
This product can be used as a control in applications like ELISA and Western Blot. It can be used in a wide range of in vitro applications, including blocking/inhibition, fibrinolysis, plasminogen activation, receptor binding, in hypertension studies, for protein-protein interactions, and more. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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