Select this product for Molecular Innovations name, IgG1, Human Myeloma Plasma and product number, HU-IGG1M. Human IgG1 from Myeloma Plasma Affinity Purified from Innovative Research has been purified from human myeloma plasma by affinity chromatography. This is a Frozen liquid buffered in 0.02M Sodium Phosphate; 0.15M NaCl; 0.05% Azide; pH 7.4 and a purity of >95% as determined by SDS-PAGE. More Details: Species: Human Target: IgG1 Purification Method: Affinity purified Purity: >95% as determined by SDS-PAGE Analysis Source: Human myeloma plasma Storage Conditions: -70 C

Additional Information:

Single arc by IEP against antisera to whole human serum, human IgG, and human kappa. Indirect ELISA indicates IgG1 subclass. This product can be used as an isotype control in applications like ELISA and Western Blot. It can also be used in a wide range of in vitro research applications. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Concentration may vary from lot to lot. Please refer to the data sheet included with your order or contact us in advance if you seek a specific concentration.

• Preheat a water bath to 37 C.
• Place the frozen plasma sample directly in the water bath.
• Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
• The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
• The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
• Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
• Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
• Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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