Human Vascular Endothelial Growth Factor 165 ELISA Kit

Human Vascular Endothelial Growth Factor 165 Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Vascular Endothelial Growth Factor 165 in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . VEGF165 present in samples reacts with an anti-Human VEGF165 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-VEGF165 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active VEGF165 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human VEGF165. Detection Target: Human Vascular Endothelial Growth Factor 165 Reactivity: Human Range: 15.6-1000pg/mL Sensitivity: 5.7pg/mL Storage Conditions: Store all components at 4┬░C This Human VEGF165 activity assay is for the quantitative determination of Vascular Endothelial Growth Factor 165 in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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This material is sold for in-vitro use only for research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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