Human tPA ELISA KitIHUTPAKT
This human tissue-type plasminogen activator (tPA) activity assay is intended for the quantitative determination of active tPA in human plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.
Functionally active tPA will form a covalent complex with biotinylated human PAI-1 which is bound to the avidin coated on the microtiter plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, polyclonal anti-human tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound polyclonal antibody is reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human tPA. The amount of color development is directly proportional to the concentration of active tPA in the sample.
- Detection Target: tPA
- Reactivity: Human
- Range: 0.01-1 IU/ml
- Sensitivity: 0.006 IU/mL
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Please note – this product replaces our old product number IHTPAKT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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