*Human Total Prekallikrein ELISA KitIHUPKKTT
Select this product for Molecular Innovations name, Human Prekallikrein Total Antigen ELISA Kit and product number, HPKKT-TOT.
This human prekallikrein total antigen assay is intended for the quantitative determination of total prekallikrein antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.
Human prekallikrein will bind to the monoclonal capture antibody coated on the microtiter plate. Prekallikrein and kallikrein will react with the antibody on the plate. After appropriate washing steps, anti human prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prekallikrein. Color development is proportional to the concentration of total prekallikrein in the samples.
- Detection Target: Prekallikrein
- Reactivity: Human
- Range: 0.02-10µg/ml
- Sensitivity: 0.012µg/ml
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Please note â€“ this product replaces our old product number IHPKKT-TOT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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