Human Total Factor VIII ELISA KitIHUFVIIIKTT
This human coagulation Factor VIII antigen assay is intended for the quantitative determination of total Factor VIII antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.
Human Factor VIII will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human Factor VIII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human plasma. Color development is proportional to the concentration of Factor VIII in the samples.
- Detection Target: Factor VIII
- Reactivity: Human
- Range: 0.0016-0.84 IU/ml
- Sensitivity: 0.00029 IU/ml
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Please note – this product replaces our old product number IHFVIIIKT-TOT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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