Human Total Factor IX ELISA KitIHUFIXKTT
This human coagulation Factor IX antigen assay is intended for the quantitative determination of total Factor IX antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.
Human Factor IX will bind to the affinity purified capture antibody coated on the microtiter plate. Factor IX and IXa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor IX primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor IX present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor IX. Color development is proportional to the concentration of Factor IX in the samples.
- Detection Target: Factor IX
- Reactivity: Human
- Range: 0.1-100 ng/ml
- Sensitivity: 0.044 ng/ml
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Please note – this product replaces our old product number IHFIXKT-TOT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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