Human Total Factor II (Prothrombin) ELISA KitIHUFIIKTT
Select this product for Molecular Innovations name, Human Prothrombin Total Antigen ELISA Kit and product number, HPTKT-TOT.
This human prothrombin antigen assay is intended for the quantitative determination of total prothrombin antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.
Human prothrombin will bind to the capture antibody coated on the microtiter plate. Prothrombin, thrombin, and thrombin-antithrombin complex will react with the antibody on the plate. After appropriate washing steps, anti human prothrombin primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human prothrombin. Color development is proportional to the concentration of total prothrombin in the samples.
- Detection Target: Prothrombin
- Reactivity: Human
- Range: 0.25-100 ng/ml
- Sensitivity: 0.078 ng/ml
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Please note â€“ this product replaces our old product number IRAPKT3100 (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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