Human sCD4 Recombinant Protein Lyophilized from Innovative Research has been recombinantly produced in CHO cells. This is a Lyophilized protein buffered in with a purity of ? 95% by SDS-PAGE gel and HPLC analyses..
More Details:
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Species: Human
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Target: CD4
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Purity: ? 95% by SDS-PAGE gel and HPLC analyses.
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Source: CHO cells
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Storage Conditions: The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Information:
Cluster determinant 4 (CD4), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction and T cell differentiation, development and activation. CD4 is constitutively expressed on the surface of various immune cells, including monocytes, macrophages, eosinophils, dendritic cells, and most prominently T lymphocytes, where it functions as an essential co-receptor and co-ligand for T cell receptor (TCR) and major histocompatibility complex class II (MHC-II) molecules. Ligation by MHC-II molecules on the surface of antigen-presenting cells can serve to influence adaptive immunity by facilitating helper T cell activation and macrophage differentiation, while ligation by proinflammatory cytokine IL-16 can contribute to innate immunity by chemoattracting CD4-expressing peripheral immune cells along an IL-16 gradient for their recruitment and activation at sites of inflammation. The protean functionality of CD4 extends past immunity as CD4 also notably serves as the major receptor for HIV-1 and human herpes virus 7 (HHV-7) infections. During HIV pathogenesis, CD4 acts instrumentally as a high-affinity entry receptor for the internalization of HIV-1 following binding of the viral envelope glycoprotein gp120 to CD4’s extracellular domain. CHO cell-derived Recombinant Human sCD4 is amonomeric glycoprotein of 371 amino acid residues, which correspond to the extracellular CD4 domain, and a calculated molecular weight of 41.3 kDa. As a result of glycosylation, Recombinant Human sCD4 migrates with an apparent molecular mass of approximately 45-55 kDa by SDS-PAGE Gel analysis, under reducing conditions.
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