Select this product for Molecular Innovations name, Human Prorenin Antigen Sandwich ELISA Kit and product number, HPRENKT. Human prorenin assay is intended for the quantitative determination of prorenin in plasma samples and serum. Active renin will not be detected by this assay. Prorenin is measured directly by Sandwich ELISA without pretreatment of samples or conversion to renin. For research use only. Strip well format. Reagents for up to 96 tests. Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Only prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prorenin. The amount of color development is directly proportional to the concentration of prorenin in the sample. More Details: Detection Target: Prorenin Reactivity: Human Range: 0.02-10 ng/ml Sensitivity: 0.013 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ŌĆō this product replaces our old product number IHPRENKT (same product, new number).
Curious About The Best Way To Thaw Plasma?
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results
Click here to view a video tutorial covering frequently asked questions regarding ELISA workflows.
Powered by Bioz
