Human Prorenin ELISA Kit for Biological FluidsIHUPRENKTBF
Human prorenin assay is intended for the quantitative determination of prorenin in non-plasma samples including cell culture media, tissue extracts, or urine samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin. For quantitation of prorenin in plasma or serum samples, use the Human Prorenin ELISA Kit (cat # HPRENKT). For research use only. Strip well format. Reagents for up to 96 tests.
Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Only prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prorenin. The amount of color development is directly proportional to the concentration of prorenin in the sample.
- Detection Target: Prorenin
- Reactivity: Human
- Range: 0.02-10 ng/ml
- Sensitivity: 0.016 ng/ml
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Please note – this product replaces our old product number IHPRENKT-NP (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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