Human Flap Endonuclease 1 Recombinant Protein Tag Free Liquid from Innovative Research has been recombinantly produced in E. coli. This is a Liquid protein buffered in Supplied as a 0.2 um filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0. It is not recommended to reconstitute to a concentration less than 100UG/ml. with a purity of Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test..More Details: Species: Human Target: FEN1 Purity: Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. Source: E. coli Storage Conditions: Store at -20░C, stable for 6 months after receipt.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Additional Information:

Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.This recombinant protein can be used for biological assays. For research use only. . At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.

View all citations and product references

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.

No Data Sheets available