Human Ferritin ELISA Kit

The Human Ferritin ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ferritin in Human biological fluids. Strip well format. Reagents for up to 96 tests. In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. More Details: Detection Target: Ferritin Reactivity: Human Range: 1.56 ng/ml - 100 ng/ml Sensitivity: 0.745 ng/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRKTAH1132 (same product, new number).