Human Factor XIa ELISA Kit

Intended Use

Innovative Research's Human Coagulation Factor XIa enzyme assay is intended for the quantitative determination of Factor XIa enzyme in human plasma and serum. For research use only.

Background
Factor XI (FXI) is a plasma zymogen involved in the intrinsic coagulation pathway. Upon proteolytic activation, FXI is converted to its active serine protease form, factor XIa (FXIa). FXI can be activated by factor XIIa (FXIIa) through the contact pathway and by thrombin-mediated feedback activation, resulting in amplification of coagulation through the activation of factor IX. Activated factor IX, together with factor VIIIa, promotes thrombin generation and subsequent fibrin formation. Because FXIa is a key mediator of coagulation amplification, measurement of circulating FXIa provides a valuable tool for evaluating activation of the intrinsic coagulation pathway. Enzyme-linked immunosorbent assays (ELISAs) have been developed for the sensitive detection and quantification of FXIa in biological samples.

Assay Principle
Functionally active FXIa will form a covalent complex with biotinylated human PAI-1 which is bound to the avidin coated on the microtiter plate. FXI will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-human XIa primary antibody binds to the captured FXIa. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human FXIa. The amount of color development is directly proportional to the concentration of FXIa in the sample.

The assay measures FXIa in the range stated below. If the unknown is thought to have higher FXIa levels, dilutions may be made in blocking buffer.

More Details

Detection Target: Factor XIa

Reactivity: Human

Range: 0.2 -100 ng/mL

Sensitivity: Calculated on a per lot basis. See Certificate of Analysis

Specificity: This assay recognizes natural and recombinant human FXIa. The assay does not capture natural or recombinant human FXI

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. For Research Use Only. Not for human or diagnostic applications.

Click here to view a video tutorial covering frequently asked questions regarding ELISA workflows.

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This material is sold for in-vitro use only for research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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