Human Collagen Type III Alpha 1 Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Collagen Type III Alpha 1 in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . COL3a1 present in samples reacts with an anti-Human COL3a1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-COL3a1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active COL3a1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human COL3a1. Detection Target: Human Collagen Type III Alpha 1 Reactivity: Human Range: 0.625-40ng/mL Sensitivity: 0.226ng/mL Storage Conditions: Store all components at 4┬░C This Human COL3a1 activity assay is for the quantitative determination of Collagen Type III Alpha 1 in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
Click here to view a video tutorial covering frequently asked questions regarding ELISA workflows.

View all citations and product references

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.

No Data Sheets available