Human Chemokine Ligand 19 (CCL19) ELISA Kit

Human Chemokine Ligand 19 (CCL19) ELISA Kit is for quantitative detection of human CCL19 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

The human CCL19 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL19 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL19 amount of sample captured in plate.

More Details:

• Detection Target: Natural and recombinant Human CCL19
• Reactivity: Human
• Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml

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Please note – this product replaces our old product number IRKTAH5040 (same product, new number).